Regionalized Brain and Spinal Cord Organoids from Human iPSCs

Researchers generated regionalized brain and spinal cord organoids from human induced pluripotent stem cells (hiPSCs) using various differentiation and culture protocols. These organoids mimicked different brain regions, including the cortex, dorsal and ventral midbrain, and spinal cord. The researchers characterized the cellular composition, gene expression, and neuronal activity of the organoids using single-cell RNA sequencing, immunocytochemistry, and calcium imaging. They further constructed assembloids – combinations of organoids – to study inter-regional connectivity. This research provides valuable in vitro models for studying human brain development and neurological diseases.