Single-Cell Sequencing Reveals Epigenetic Remodeling in White Adipose Tissue of Obese Mice and Humans

This study employed single-cell RNA sequencing (snRNA-seq), CUT&TAG, and ATAC-seq to investigate white adipose tissue (WAT) in obese mice and humans, revealing significant alterations in the epigenetic landscape of WAT cell types during weight loss. A portion of gene expression changes persisted, suggesting an epigenetic memory mechanism of obesity. Researchers analyzed samples from three independent human studies (MTSS, LTSS, and NEFA) and a diet-induced obesity mouse model. Multi-omics analysis revealed changes in WAT cell types and their association with metabolic function.